Transcriptome analysis in blastocyst hatching by cDNA microarray*

Transcriptome analysis in blastocyst hatching by cDNA microarray.

BACKGROUND Hatching is an important process for early embryo development, differentiation and implantation. However, little is known about its regulatory mechanisms. By integrating the technologies of RNA amplification and cDNA microarrays, it has become possible to study the gene expression profile at this critical stage. METHODS Pre-hatched and hatched ICR mouse embryos (25 blastocysts in e...

cDNA Microarray Data Normalization

Analysis of CDNA Microarray Images

Microarrays are part of a new class of biotechnologies that allow the monitoring of expression levels for thousands of genes simultaneously. Image analysis is an important aspect of microarray experiments, one that can have a potentially large impact on subsequent analyses, such as clustering or the identification of differentially expressed genes. This paper reviews a number of existing image ...

Statistical issues in cDNA microarray data analysis.

Statistical considerations are frequently to the fore in the analysis of microarray data, as researchers sift through massive amounts of data and adjust for various sources of variability in order to identify the important genes amongst the many which are measured. This article summarizes some of the issues involved and provides a brief review of the analysis tools which are available to resear...

Assessment of gene expression profile by cDNA microarray analysis.

With the advent of cDNA microarray technology, variations in cellular transcriptional events can now be monitored simultaneously, and expression profile at each functional state can be established and compared to one another. Here we illustrate gene expression profile analysis of a cisplatin-resistant ovarian carcinoma cell line (A2780CP)and its parental A2780 cell line, and compare them to ass...